Enzo Life Sciences的ROS-ID® NO Detection kit以紅色熒光的NO檢測(cè)試劑為主要成分，可通過(guò)熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活細(xì)胞中NO的產(chǎn)生。這種非熒光的、可滲透細(xì)胞的NO檢測(cè)染料在O2存在的情況下能夠與NO發(fā)生反應(yīng)，具有高特異性、靈敏性和準(zhǔn)確性，產(chǎn)生不溶于水的紅色熒光產(chǎn)物。這種染料對(duì)過(guò)氧亞硝酸鹽沒(méi)有反應(yīng)性，因此可以區(qū)分過(guò)氧亞硝酸鹽和一氧化氮。

ROS-ID® NO Detection kit中的熒光探針對(duì)活性氯或溴不敏感，因此不用于檢測(cè)這些分析物。染色后，該染料產(chǎn)生的熒光產(chǎn)物可通過(guò)熒光顯微鏡進(jìn)行觀(guān)察。當(dāng)需要同時(shí)檢測(cè)額外的熒光信號(hào)（綠色或橙色）時(shí)，建議使用650/670 nm的組合。ROS-ID® NO Detection kit包含足夠的試劑，可對(duì)活細(xì)胞（貼壁或懸?。┻M(jìn)行至少200次檢測(cè)。

產(chǎn)品特色

通過(guò)熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活體細(xì)胞中NO的產(chǎn)生

高特異性、靈敏性和準(zhǔn)確性，產(chǎn)生不溶于水的紅色熒光產(chǎn)物

可區(qū)分過(guò)氧亞硝酸鹽和一氧化氮

試劑盒內(nèi)包含一氧化氮誘導(dǎo)劑和清除劑

產(chǎn)品信息

部分產(chǎn)品引用文獻(xiàn)

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12. Ginsenoside Rg3 regulates S-Nitrosylation of the NLRP3 inflammasome via suppression of iNOS: S. J. Yoon, et al.; Biochem. Biophys. Res. Commun. 463, 1184 (2015)

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15. Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia: Y. Yuan, et al.; BMC Neurosci. 15, 125 (2014)

16. Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism: Z.G. Khalil, et al.; Mycology 5, 168 (2014)

17. Cyclosporine attenuates arginine transport, in human endothelial cells, through modulation of cationic amino acid transporter-1: A. Grupper, et al.; Am. J. Nephrol. 37, 613 (2013)

18. Identification and characterization of a functional mitochondrial angiotensin system: P.M. Abadir, et al.; PNAS 108, 14849 (2011)

19. Loss of bcl-2 during the senescence exacerbates the impaired angiogenic functions in endothelial cells by deteriorating the mitochondrial redox state: M. Uraoka, et al.; Hypertension 58, 254 (2011)

詳情請(qǐng)聯(lián)系Enzo Life Sciences金牌代理——欣博盛生物